If you have the patient’s absolute lymphocyte count (ALC), call 513-636-4685 or use the "Customized Volumes by ALC"sheet to obtain the precise amount of blood required for this assay. If you do not have an ALC available, 5-10mL blood in Sodium Heparin (Dark Green) is the general requirement and should be sufficient for patients who are not lymphopenic.
Minimum Collection Volume
Minimum volume dependent on ALC
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Specimens collected in anticoagulants other than Sodium Heparin. Specimens received greater than 24 hours after collection.
Storage/Transport Temperature
CRITICAL AMBIENT
Specimen Preparation
Transport whole blood in original collection tubes. Do not refrigerate or freeze. Specimen must be received by our laboratory within 24 hours of collection and before 1500 M-Th and before 1400 on Fri. LIVE LYMPHOCYTES REQUIRED.
The complex cascade of events that comprise the cellular portion of the immune response include the proliferation of lymphocytes in response to upstream events (activation, Ca++ flux, etc.). This assay tests the capacity of lymphocytes to proliferate in response to exposure to mitosis-inducing agents (mitogens). Specifically, peripheral blood mononuclear cells are exposed to phytohemagglutinin (PHA), concanavalin A (ConA), and pokeweed mitogen (PWM), and cultured for several days. An excess of radio-labeled thymidine is made available to the cells during the later part of the culture period. As the stimulated cells enter the S phase of the cell cycle, they synthesize DNA, incorporating the radio-labeled thymidine which is then quantitated using a microplate scintillation counter. Results are expressed as counts per minute (cpm).
CPT Codes
86353x3
Collection
Notes
This test is New York DOH approved.
Collect
If you have the patient’s absolute lymphocyte count (ALC), call 513-636-4685 or use the "Customized Volumes by ALC"sheet to obtain the precise amount of blood required for this assay. If you do not have an ALC available, 5-10mL blood in Sodium Heparin (Dark Green) is the general requirement and should be sufficient for patients who are not lymphopenic.
Minimum Collection Volume
Minimum volume dependent on ALC
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Specimens collected in anticoagulants other than Sodium Heparin. Specimens received greater than 24 hours after collection.
Storage/Transport Temperature
CRITICAL AMBIENT
Specimen Preparation
Transport whole blood in original collection tubes. Do not refrigerate or freeze. Specimen must be received by our laboratory within 24 hours of collection and before 1500 M-Th and before 1400 on Fri. LIVE LYMPHOCYTES REQUIRED.
The complex cascade of events that comprise the cellular portion of the immune response include the proliferation of lymphocytes in response to upstream events (activation, Ca++ flux, etc.). This assay tests the capacity of lymphocytes to proliferate in response to exposure to mitosis-inducing agents (mitogens). Specifically, peripheral blood mononuclear cells are exposed to phytohemagglutinin (PHA), concanavalin A (ConA), and pokeweed mitogen (PWM), and cultured for several days. An excess of radio-labeled thymidine is made available to the cells during the later part of the culture period. As the stimulated cells enter the S phase of the cell cycle, they synthesize DNA, incorporating the radio-labeled thymidine which is then quantitated using a microplate scintillation counter. Results are expressed as counts per minute (cpm).
